Fig. 2
From: CXCR4 peptide-based fluorescence endoscopy in a mouse model of Barrett’s esophagus
Ex vivo wide-field imaging shows accumulation of CXCR4-targeted MK007 in dysplastic lesions of IL1B mice. A Representative ex vivo fluorescence imaging of three IL1B mice injected with MK007 (left) and one non-injected 12-month-old IL1B mouse (right, upper row), which had a dysplasia score of 3. The different dysplasia score (1–3) is reported. The quantification of ex vivo Sulfo-Cy5 signal by CTCF in mouse stomach and organs 4 h post-injection showed peptide accumulation also in liver, kidney, and spleen (right, middle row). Target-to-background ratio (TBR) of injected mice was 2.45 ± 0.24 compared with negative control (1.26). TBR was calculated as reported in Methods. Data in both graphs are represented as mean ± SEM (n = 5). B Representative confocal images of areas from squamocolumnar junction (SCJ), squamous, and glandular epithelium of the IL1B mouse with dysplasia score 2 in 2A showed less or no Sulfo-Cy5 signal in non-dysplastic areas than dysplastic areas (arrows). Sulfo-Cy5 signal was detected by using Cy5.5 red channel. Additionally, most CXCR4 + cells were detected in the SCJ and squamous epithelium (Squamous Ep.) of IL1B mice, compared with the normal glandular epithelium (Glandular Ep.). Scale bars represent 50 μm