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Table 2 Challenges and benefits of interdisciplinary cooperation between researchers and pathologists in translational oncology research

From: Correlation of in vivo imaging to morphomolecular pathology in translational research: challenge accepted

Scientific step Coordinator Challenges/solutions Benefit for researchers
Project management Researcher Definition of question, aim of the study, study and experimental design  
Pathologist Experimental design with focus on appropriate mouse models and histopathological evaluation with focus on co-localization Choosing a suitable animal model for the research question, appropriate/realistic time table
In vivo imaging Researcher Performance, coordination of procedure and time table in consultation with pathologist  
Pathologist Coordination of histopathological tissue processing after in vivo imaging according to time table of researcher Fast and correct tissue sampling and processing
Submission of tissue/animals Researcher Inform pathologist before necropsy, planning of necropsy in consultation with pathologist  
Pathologist Feedback to researcher about time table, fixation, tissue processing with focus on co-localization, provide appropriate protocols for necropsy Fast and correct tissue sampling and processing
Co-localization
- Basic co-localization Researcher Definition of question and aim of co-localization approach  
Planning of necropsy with focus on special orientation  
Pathologist Planning and consulting of necropsy and tissue processing
- Flagging by ink
- Embedding in the right plane
- Consecutive slides
Ensure the correct orientation of tissues for later co-localization
- Complex co-localization Researcher Definition of question and aim of co-localization approach  
Planning of necropsy with focus on special orientation  
Pathologist Planning/consulting of necropsy/tissue processing with focus on orientation
- Removement of tissue with adjacent organ structures
- Flagging by ink
- Embedding in the right plane
- Consecutive slides
Ensure the correct orientation of tissues for later co-localization
Tissue processing Researcher In consultation with pathologist  
Pathologist Time table and coordination with the pathology laboratory (e.g. CEP)
- Fixation
- Embedding in the right plane, special orientation
- Cutting: number and type of slides, consecutive slides
- Staining: H&E, additional special stainings
Fast and appropriate tissue processing
- Immunohistochemistry Selection of the right target antigen, antibody, protocol
- Scanning Simplification of co-localization and co-registration
Histopathological evaluation Researcher In consultation with pathologist  
Pathologist Evaluation of H&E stains, special stainings, immunohistochemistry Correct histopathological diagnosis because of
- Classification of lesions in a comparative way
- Standardized vocabulary in description
- Knowledge of strain-specific background lesions
- Knowledge of differential diagnoses (non-expected alterations)
Co-registration Researcher Combining in vivo imaging with histopathological findings, in consultation with pathologist  
Pathologist Consulting researcher with focus on co-localization of histomorphological findings to in vivo imaging Use of correct histopathological terms and localization for description of findings
Summary Interdisciplinary cooperation Accuracy and reproducibility of results