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Fig.1 | EJNMMI Research

Fig.1

From: Thrombocytopenia after meta-iodobenzylguanidine (MIBG) therapy in neuroblastoma patients may be caused by selective MIBG uptake via the serotonin transporter located on megakaryocytes

Fig.1

mRNA expression of SERT and NET in megakaryocytic cells evaluated using RT-qPCR. a mRNA expression of SERT and NET in CD34+ HSPCs undergoing megakaryocytic differentiation. Cells were cultured for 14 days, SERT and NET mRNA expression was evaluated every two days. b SERT mRNA expression of megakaryocytic cell culture batches from three different HSPC donors (MK1-3) after 10 days of differentiation. HEK-EV, HEK-NET, HEK-SERT are HEK293 cells were transfected with an empty vector, NET, or SERT, respectively, and served, in combination with fibroblasts (FB) as controls. c NET mRNA expression of MK1-3 after 10 days of differentiation. NET mRNA expression was also determined in fibroblasts (FB) and HEK293-EV, HEK293-NET, and HEK293-SERT. Results are expressed as mean ± SD (n = 2, experiments in triplicate). *If a ΔCT could not be calculated due to CT values of SERT or NET > 40 cycles, the sample was scored ‘Negative’

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