Skip to main content

Table 1 Fluorescently labelled P0 peptides, sequences and outcome synthesis

From: Intraoperative visualization of nerves using a myelin protein-zero specific fluorescent tracer

Peptide Amino acid sequence Negative/positive charges Solubility in H2O (µM) KD in nM
Cy5-P01–25 H-IVVYTDREVHGAVGSQVTLHC(Cy5)SFWS-NH2 4+/4− 12  > 1000
Cy5-P041–65 Ac-PEGGRDAISIFHYAKGQPYIDEVGTC(Cy5)-NH2 3+/7− 45  > 1000
Cy5-P061–85 Ac-DEVGTFKERIQWVGDPRWKDGSIVIC(Cy5)-NH2 5+/8− 6  > 1000
Cy5-P081–105 Ac-GSIVIHNLDYSDNGTFTC(Cy5)DVKNPPD-NH2 2+/6− 68  > 1000
Cy5-P095–120 Ac-TFTADVKNPPDIVGKTSQVTLYVFEKC(Cy5)-NH2a 4+/5− 150  > 1000
Cy5-P0101–125 Ac-KNPPDIVGKTSQVTLYVFEKVPTRYC(Cy5)-NH2a 4+/6− 172 105 ± 17
  1. aItalic alanine residue replacing the cysteine from native P0, bolded and underlined residues were implemented via the above-mentioned pseudoproline method [34], underlined cysteines were non-native residues added to the C-terminus