Intraoperative visualization of nerves using a myelin protein-zero specific fluorescent tracer

EJNMMI Research

Table 1 Fluorescently labelled P0 peptides, sequences and outcome synthesis

Peptide	Amino acid sequence	Negative/positive charges	Solubility in H₂O (µM)	K_D in nM
Cy5-P0_1–25	H-IVVYTDREVHGAVGSQVTLHC(Cy5)SFWS-NH₂	4+/4−	12	> 1000
Cy5-P0_41–65	Ac-PEGGRDAISIFHYAKGQPYIDEVGTC(Cy5)-NH₂	3+/7−	45	> 1000
Cy5-P0_61–85	Ac-DEVGTFKERIQWVGDPRWKDGSIVIC(Cy5)-NH₂	5+/8−	6	> 1000
Cy5-P0_81–105	Ac-GSIVIHNLDYSDNGTFTC(Cy5)DVKNPPD-NH₂	2+/6−	68	> 1000
Cy5-P0_95–120	Ac-TFTADVKNPPDIVGKTSQVTLYVFEKC(Cy5)-NH₂^a	4+/5−	150	> 1000
Cy5-P0_101–125	Ac-KNPPDIVGKTSQVTLYVFEKVPTRYC(Cy5)-NH₂^a	4+/6−	172	105 ± 17

^aItalic alanine residue replacing the cysteine from native P0, bolded and underlined residues were implemented via the above-mentioned pseudoproline method [34], underlined cysteines were non-native residues added to the C-terminus