Fig. 4
From: Intraoperative visualization of nerves using a myelin protein-zero specific fluorescent tracer
Ex vivo and in vivo imaging of the nervus ischiadicus of mice. A 63 × magnification of the nervus ischiadicus after (I) ex vivo incubation, (II) intraneural administration or (III) intravenous (v. femoralis) administration of Cy5-P0101–125. (IV) Fluorescence confocal image of the nervus ischiadicus F after intravenous (v. femoralis) administration of Cy5-NP41 or (V) when no tracer was applied. B In vivo fluorescence confocal image of a large field of view of the nervus ischiadicus of a THY-1 YFP at 1 h after intravenous (v. femoralis) administration of Cy5-P0101–125. Cy5 in red and intrinsic YFP located in the axons of THY-1 YFP mice in green. C In vivo Dinolight microscopy images showing (I) a whitelight image of the nervus ischiadicus and (II) the intrinsic YFP signal in a THY-1 YFP mouse. Image processing after illumination of the nervus ischiadicus after intraneural administration of Cy5-P0101–125 in (III) a THY-1 YFP mouse, (IV) a non-YFP Balb/c nude mouse and (V) when no tracer was applied. Inserts show unprocessed fluorescence image. Scalebar represents the nerve-to-background ratio (NBR)