Fig. 2

RBC-mediated quenching of Fc-AMBF₃ but not IR783-AMBF₃ fluorescence. Fluorescence imaging of different concentrations of IR783-AMBF₃ or Fc-AMBF₃ in PBS, a, or when mixed with 1.5x10⁹ RBC/per tube, b. When mixed with blood, Fc-AMBF₃ fluorescence is not visible, while IR783-AMBF₃ fluorescence is clearly visible. Fluorescence imaging of blood contaminated CSF-containing rhinorrhea following the creation of a CSF leak using a 22G needle c. Excitation/emission filters for Fc-AMBF₃ and IR783-AMBF₃ were set at 450/535 nm and 760/830 nm, respectively. Note that in a CSF leak where RBC is not present, Fc-AMBF₃ is visible by fluorescence [20]