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EJNMMI Research
Fig. 7 | EJNMMI Research

Fig. 7

From: Indium-111-labeled CD166-targeted peptide as a potential nuclear imaging agent for detecting colorectal cancer stem-like cells in a xenograft mouse model

Fig. 7

The synthesis and stability assay for 111In-DTPA-CD166tp-G18C. a The flowchart of 111In-DTPA-CD166tp-G18C synthesis. CD166tp-G18C was conjugated with DTPA for indium-111 labeling and then intravenously injected into CD166+HCT15 xenograft mice for CD166 detection in tumor area. b The molecular weights of DTPA-CD166tp-G18C detection. The molecular weights of Maleimide-DTPA, CD166-G18C, and DTPA-CD166tp-G18C were confirmed by a mass spectrometry. c The labeling efficiency of 111In-DTPA-CD166tp-G18C. After indium-111 labeling, instant thin-layer chromatography (ITLC) was performed to determine the radio-labeling efficiency in 111In-DTPA-CD166tp-G18C. d The stability of 111In-DTPA-CD166tp-G18C. The radioactivity of 111In-DTPA-CD166tp-G18C was detected in human, fetal bovine, and mouse serum for 144 h at 37 °C. The radioactivity was detected by ITLC. Data are presented as mean ± SD (n = 5)

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