Fig. 1

Isolation and analysis of CD166⁺HCT15 cells. a Schematic diagram for the isolation of CD166⁺HCT15 cells by magnetic-activated cell sorting (MACS). b The rate of CD166 expression in HCT15 cell sorted population determined by flow cytometry. Both CD166⁺HCT15 and CD166⁻HCT15 cells were treated with IgG-FITC and FITC-labeled CD166 antibody (CD166ab-FITC, 20 μg/mL) for 1 h. Data are presented as mean ± SD (n ≥ 3). *P < 0.05, versus IgG-FITC. c The protein levels of CD166 in both CD166⁺HCT15 and CD166⁻HCT15 cells. The protein expression was detected by Western blotting and quantified by densitometry and normalized by GAPDH levels. The data are presented as mean ± SD (n ≥ 3). *P < 0.05, versus CD166⁻HCT15