Fig. 2

a Binding affinities (IC₅₀ in nM, 1 h, 4 °C) of [¹⁹F, ^natGa]rhPSMA-7.1 to -7.4 (white; n = 5–9) and the references diastereomeric mixture [¹⁹F, ^natGa]rhPSMA-7 (grey; n = 3), [¹⁹F]DCFPyL and [¹⁹F]PSMA-1007 (black; n = 3); b internalized activity of [¹⁸F, ^natGa]rhPSMA-7.1 to -7.4 (white; n = 3–6) and the references diastereomeric mixture [¹⁹F, ⁶⁸ Ga]rhPSMA-7 (grey; n = 3), [¹⁸F]DCFPyL and [¹⁸F]PSMA-1007 (black; n = 3) in LNCaP cells (1 h, 37 °C) as a percentage of the reference ligand ([¹²⁵I]I-BA)KuE); c lipophilicity of [¹⁸F, ^natGa]rhPSMA-7.1 to -7.4 (white; n = 18–23) and the references diastereomeric mixture [¹⁸F, ^natGa]rhPSMA-7 (grey; n = 13), [¹⁸F]DCFPyL and [¹⁸F]PSMA-1007 (black; n = 3), expressed as n-octanol/PBS (pH 7.4) distribution coefficient (log D); d human serum albumin binding of [¹⁹F, ^natGa]rhPSMA-7.1 to -7.4 (white) and the references diastereomeric mixture [¹⁹F, ^natGa]rhPSMA-7 (grey), [¹⁹F]DCFPyL and [¹⁹F]PSMA-1007 (black), determined on a Chiralpak HSA column. Data for the reference ligands were [15, 19] taken from a previously published studies conducted by our group. Values are expressed as mean ± standard deviation