Fig. 4

PET images of ¹⁸F-C2Am in tumor-bearing mice, pre- and post-treatment. Maximum intensity projections of the PET signal in sagittal sections of representative mice, bearing MDA-MB-231 (a) and Colo205 (b) tumors. Projections are shown from immediately before (0) and up to 120 min after injection of ¹⁸F-C2Am. Signal is shown as injected activity per gram of tissue (IA/g, %) and overlaid on a CT-derived skeleton mask. White arrows indicate tumor location. Time–activity (IA/g, %) curves for the indicated tissues in treated (MEDI3039, 0.4 mg/kg, 24 h) MDA-MB-231 (c) and Colo205 (d) tumor-bearing mice. Also shown are tumor-to-blood (T/b) and tumor-to-muscle (T/m) ratios for both tumor models. e, f Pairwise analysis of PET signal pre- and 1 h (first and third columns) or 2 h (second and fourth columns) post-treatment, expressed as IA/g (%) (e) or SUV (f). Pairwise analysis (g) of tumor bioluminescence pre- and post-treatment (first and third plots) and correlation analysis (Pearson) of tumor PET signal with the reduction in BLI signal (second and fourth plots), 1 h after ¹⁸F-C2Am administration. Data (c, d) is mean ± SEM, n = 5−6 per treatment group. Black and red symbols (e, g) correspond to MEDI3039 treatment at 0.4 or 0.1 mg/kg (for 24 h), respectively. e, g Two-tailed, pairwise t test, unequal variance. *P < 0.05, **P < 0.01