Table 4 Differences and similarities between radio-HPLC and TLC-autoradiography
From: Plasma radio-metabolite analysis of PET tracers for dynamic PET imaging: TLC and autoradiography
Properties | Radio-HPLC | TLC—autoradiography |
|---|---|---|
Volume of plasma required | 1–2000 \(\upmu \mathrm{L}\) [31] | 2 \(\upmu \mathrm{L}\) |
Mobile-phase optimization | Required | Required |
Analyte resolution | High | Low—overlap of analyte peaks can be resolved by Gaussian fitting |
Analyte identificationa | Yes | No |
Sample analysis mode | Serial | Multiplex |
Operation | May require separate HPLC column for each tracer | Single use inexpensive TLC plate for each run |
HPLC column requires regular washing to prevent clogging and removal residual analytes from previous runs [1, 32] | Single use TLC—no maintenance is required | |
Tissue section autoradiography | No | Yes |