Fig. 6
Evaluation of glucose metabolism and redox status in the cardiac myocardium of SOD1G93A and control mice. a Average of FDG retention expressed as standardized uptake value ratio (SUVr) in control (green column) and SOD1G93A myocardium (red column). Data are expressed as mean ± SD, n = 5 for each group. b Average time-course of “ex-vivo” radioactivity expressed as a fraction of the FDG dose, measured by the Ligand-Tracer White® apparatus, in myocardium harvested from control (green line) and SOD1G93A mice (red line). c–f Myocardial catalytic activities of HK, PFK, G6PD, H6PD, and GR (g) in control (green column) and SOD1G93A (red column). h MDA levels and i MFI H2DCFDA in control and SOD1G93A experimental groups. j, k Immunofluorescence representative images of Mitotracker® (as specific mitochondria staining—red fluorescence), H2DCFDA (as specific ROS staining—green fluorescence), and colocalization staining signals, in control and SOD1G93A myocardium. Data are expressed as mean ± SD, n = 3 for each group. Student t test for unpaired data was used for statistical evaluation