Fig. 3
Effect of SOD1G93A mutation on enzymatic pathway and colocalization of 2-NBDG and ER signals in harvested quadriceps. a–d Respective activities of HK, PFK, G6PD, and H6PD in control (green column) and SOD1G93A groups (red column) of quadriceps homogenate. e Immunofluorescence representative images of ER (red fluorescence), 2-NBDG (green fluorescence), and colocalization staining signals (% of 2-NBDG voxels within the ER staining—white) in control and SOD1G93A quadriceps. Blue color reports the Hoechst 33342 signal. f, g Percent voxels over threshold of ER and 2-NBDG, respectively, in control and SOD1G93A quadriceps. h Percent of ER voxels within 2-NBDG staining. i Percent of 2-NBDG voxels within ER staining. Data are expressed as mean ± SD, n = 3 for each group. Student t test for unpaired data was used for statistical evaluation