Fig. 8
From: PET imaging of soluble epoxide hydrolase in non-human primate brain with [18F]FNDP
Metabolism of [18F]FNDP in mouse blood and brain. Representative HPLC chromatograms recorded for non-radioactive FNDP standard (blue), [18F]FNDP standard (red) and blood plasma and brain extract samples obtained from mice injected with 1 mCi of [18F]FNDP and sacrificed at 10 and 30 min after radiotracer injection (red). The offset (~ 0.5 min) between UV and radiation peak retention times is because the UV and radiation detectors are connected in series. a, b, c, and d Plasma samples are analyzed with inclusion of a capture column. e, f, g and h Brain extracts are analyzed with no capture column. The study demonstrated presence of two major radiometabolites (Met-1 and Met-2) in the mouse plasma, but only a small amount of radiometabolite Met-1 entered the mouse brain