Fig. 3

Sequential 4FMFES and ⁸⁹Zr-T PET imaging on mice subcutaneously implanted with MCF-7 and JIMT-1 tumors. MIP image of a representative JIMT-1 (red arrows) and MCF-7 (blue arrows) of a tumor-bearing mice injected with 4FMFES or b with ⁸⁹Zr-T and imaged at 48 h p.i. and c at 144 h p.i. PET-derived uptake of relevant organs in %IA/g (upper graphs) and tumor-to-muscle ratios (lower graphs) for d 4FMFES, e⁸⁹Zr-T imaged at 48 h p.i., and f⁸⁹Zr-T imaged at 144 h p.i. showing the phenotype-discerning capacity of the dual-tracer approach. *p < 0.05; **p < 0.01; ***p < 0.005; ****p < 0.001