Fig. 2
From: Fluorescence labeling of a NaV1.7-targeted peptide for near-infrared nerve visualization
Chemical characterization of Hs1a-FL. a RP-HPLC chromatograms of Hs1a (black) and Hs1a-FL (pink) with absorbances observed at 280 nm. b LC-MS spectrum of Hs1a and c of Hs1a-FL. The mass spectra show four major ion species that correspond to the calculated mass of Hs1a peptide and four major ion species that confirm the calculated mass of Hs1a-FL after dye conjugation. d Fluorescence spectra (Ex/Em 720/835 nm) of 0.1 μM Hs1a peptide (black) and 0.1 μM Hs1a-FL (pink)