Table 2 Apoptosis analysis of MDA-MB-231(a) and MDA-MB-468(b) cells after incubation with treatment at 10xIC50 for 24 h, 48 h, and 72 h by flow cytometry using Annexin V/PI apoptosis assay. Results are shown as mean ± SD (n = 3 independent experiments)
MDA-MB-231 | |||||||
|---|---|---|---|---|---|---|---|
| Â | Untreated | Epirubicin | Paclitaxel | ||||
| Â | 24 h | 48 h | 72 h | 72 h | 24 h | 48 h | 72 h |
Live cells (%) | 93.8 ± 2.5 | 94.5 ± 1.7 | 94.7 ± 2.2 | 2.6 ± 1.3 | 71.8 ± 6.8 | 59.9 ± 8.0 | 50.7 ± 13.3 |
Apoptotic cells (%) | 3.3 ± 1.9 | 3.4 ± 0.4 | 2.0 ± 0.9 | 32.9 ± 1.2 | 10.5 ± 2.0 | 18.6 ± 2.5 | 28.6 ± 7.9 |
Necrotic cells (%) | 2.9 ± 0.9 | 5.1 ± 1.4 | 3.2 ± 1.3 | 64.4 ± 1.7 | 23.7 ± 1.6 | 21.5 ± 6.6 | 20.7 ± 6.2 |