Fig. 5

[¹⁸F]ML-10 uptake. a [¹⁸F]ML-10 was added to the cell suspension containing adherent cells 72 h after paclitaxel and incubation for 15 min and its uptake was determined. Results are shown as mean ± SD (n = 3 independent experiments). Mann-Whitney test was performed (***p < 0.001). b Correlation between the percentage of apoptotic cells and [¹⁸F]ML-10 binding amount in paclitaxel-treated MDA-MB-468 cells. The percentage of cells in apoptosis was determined using Annexin V/PI staining and expressed as the proportion of Annexin V-positive cell counts in total counts. Pearson correlation was performed (**p < 0.01). c Effect of acidification on [¹⁸F]ML-10 uptake. pH dependence of [¹⁸F]ML-10 uptake, with an extracellular pH ranging from of 5.9 to 8.0.