Fig. 3

Apoptotic induction after treatment by paclitaxel or epirubicin in MDA-MB-468 cells. a Time-lapse images detection of apoptosis in MDA-MB-468 cells in the presence of Caspase-3/7 Green Apoptosis reagent and Annexin V Red Reagent. b Percent of apoptotic cells with high annexin V reagent was assessed in time-lapse imaging (IncuCyte) over 72 h. c Western blot analysis showing the cleavage of PARP and Caspase 3. Actin was used as a loading control. d Quantitative analysis of apoptotic cells measured by flow cytometry in untreated, epirubicin-treated, or paclitaxel-treated MDA-MB-468 cells for 72 h. (n = 3 independent experiments; mean ± SD). Compared to untreated, ***p < 0.001 (Mann-Whitney test)