Fig. 4

Ex vivo parameters at time-of-sacrifice (i.e., a tumor volume of 1500 mm³). a Proliferation as assessed by the percentage of Ki67-positive nuclei. b Representative Ki67-stained tumor tissue of a metformin (MET)-treated animal. Arrows indicate Ki67-positively staining nuclei. c Apoptosis as assessed by the percentage of cleaved caspase-3 (CC3)-positive fragments. d Representative CC3-stained tumor tissue of a metformin-treated animal. Arrows indicate CC3-positively staining apoptotic fragments. Statistical significance was not reached. RT, radiotherapy