Fig. 4

In vivo biodistribution of apoptotic radiotracers in response to death-switch induction. a ¹⁸F-ICMT-11 and b ¹⁸F-ML-10. Biodistribution of the radiotracers was assessed in the B16ovaRevC3 tumour model, 24 h after vehicle-control or doxycycline administration (n = 6 mice per group). Radioactivity in select tissues was measured by ex vivo gamma counting and biodistribution was calculated as percentage of injected dose per gram of tissue (%injected dose/g). Tumour uptake of ¹⁸F-ICMT-11 was significantly higher after doxycycline administration (caspase-3 activation) compared with controls (*p < 0.01, Student’s t test; p = 0.02, two-way ANOVA); however, no significant difference was observed with ¹⁸F-ML-10. There were no significant differences in the tissue distribution of both radiotracers between control and doxycycline-treated mice