Fig. 2

Cellular internalization and GCGR affinity of [⁶⁸Ga]Ga-DO3A-S01-GCG and [⁶⁸Ga]Ga-DO3A-S02-GCG. Binding and internalization in HEK293 cells overexpressing human GCGR of [⁶⁸Ga]Ga-DO3A-S01-GCG (a) and [⁶⁸Ga]Ga-DO3A-S02-GCG (b). Internalization was suppressed by cooling cells to 4 °C. Affinity was measured in HEK293 cells overexpressing human GCGR by saturation binding studies on viable cells (c, d) or by in vitro autoradiography (ARG) on sections of cell pellet (e)