Fig. 3

Microscopic slides of tumours for the same mouse as for Fig. 2a, b with pimonidazole staining (× 50 magnification). Hypoxic regions are shown either in green (a fluorescence microscopy) or in brown (b transmitted light microscopy). c Correlation between Cherenkov luminescence imaging following the injection of FMISO and fluorescence pimonidazole staining (n = 9, Pearson’s coefficient = 0.7238, p < 0.05*). Results are presented as radiance (p/s/cm²/sr) for CLI and fluorescence intensity (arbitrary units, AU) of pimonidazole