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Fig. 6 | EJNMMI Research

Fig. 6

From: Bone marrow cell homing to sites of acute tibial fracture: 89Zr-oxine cell labeling with positron emission tomographic imaging in a mouse model

Fig. 6

GFP+ donor cells are detected in the bone fracture. a, b BM cells from GFP transgenic mice were transferred to wild-type mice immediately after tibial fracture and 1.5 weeks later, the fractured tibia section and corresponding contralateral tibia section were harvested. Tissues slices were stained with H&E and with GFP-IHC. Microscopic images of fractured tibia indicated that the fractured bone was surrounded by inflammatory tissue (a, b, left, H&E) and GFP+ donor cells were engrafted around the fracture (a, b, right, GFP-IHC). c, d Tissue sections created at 7 weeks following the bone marrow cell transfer indicated callus formation with GFP+ cells in the surrounding soft-tissue (left: H&E, right: GFP-IHC). e, f Contralateral non-fractured tibia at 1.5 weeks after the cell transfer is shown. g, h Tibia from a GFP+ (g) and a wild-type (h) mice are shown as controls. Scale bar indicates 200 μm in (a, c) and (e), and 100 μm in (b, d, f, g) and (h)

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