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Fig. 5 | EJNMMI Research

Fig. 5

From: Bone marrow cell homing to sites of acute tibial fracture: 89Zr-oxine cell labeling with positron emission tomographic imaging in a mouse model

Fig. 5

Flow cytometry analysis demonstrates that the donor BM cells homing to the fracture site are mostly hematopoietic cells stem/progenitor cells and inflammatory cells. a BM cells from GFP transgenic mice were used as donor cells in d1- and d0-fracture models. One day later, cells were harvested from the fractured section of the tibia, corresponding contralateral tibia section, BM from non-fracture side femur, and spleen (n = 4). Flow cytometry analysis indicated that almost all the GFP+ cells found in these tissues expressed CD45 (representative data). b The fraction of Lin cells in GFP+ donor cells is indicated (representative data). cg Comparison of cell fractions found in the sections of fractured vs non-fractured tibia is shown. CD45+ cells (c) and Lin cells (d) within the donor cells, CD29+CD105+ cells within non-hematopoietic donor cells (e), Ly6Cintermediate (int) Ly6G+ cells (f), and Ly6C+Ly6G- cells (g) within CD11b+ donor cells were analyzed. Ly6CintLy6G+ cells migrated to the fracture significantly higher than the non-fractured tibia (n = 4 in each group, *p < 0.05 with Mann-Whitney test)

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