Fig. 2
From: Leveraging PET to image folate receptor α therapy of an antibody-drug conjugate
Synthesis and characterization of [89Zr]Zr-DFO-M9346A. a Conjugation of p-isothiocyanatobenzyl-desferrioxamine (DFO-Bz-NCS) to humanized antibody M9346A in basic aqueous conditions at 37 °C for 90 min and 89Zr-radiolabeling of DFO-M9346A at 37 °C for 60 min. b After purification of reaction mixture using a PD-10 desalting column, quality control of [89Zr]Zr-DFO-M9346A was performed using radio-instant thin layer chromatography in a solution of EDTA (50 mM). c In vitro uptake studies of either [89Zr]Zr-DFO-M9346A (100 ng) or a mixture of [89Zr]Zr-DFO-M9346A (100 ng) and M9346A (10 μg) using cancer cell lines KB, OV90, H2110, and A549 with various expression levels of FRα. **P < 0.01; ***P < 0.001. Error bars represent the SD