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Fig. 2 | EJNMMI Research

Fig. 2

From: Uniform intratumoral distribution of radioactivity produced using two different radioagents, 64Cu-cyclam-RAFT-c(-RGDfK-)4 and 64Cu-ATSM, improves therapeutic efficacy in a small animal tumor model

Fig. 2

a Well-matched intratumoral distribution of 64Cu-RaftRGD and Cy5.5-RaftRGD. Mice bearing U87MG tumors were pre-injected with 37 MBq of 64Cu-RaftRGD, injected 10 min later with 20 nmol of Cy5.5-RaftRGD, and euthanized at 3 h p.i. Autoradiography and fluorescence imaging were sequentially performed in the same tumor section. Left panels, 64Cu autoradiograms; right panels, Cy5.5 fluorescence acquired using the Odyssey CLx near-infrared fluorescence imaging system. bg A representative tumor section showing distribution of 64Cu-ATSM and Cy5.5-RaftRGD. U87MG tumor-bearing mice were co-injected with 64Cu-ATSM and Cy5.5-RaftRGD and euthanized at 3 h p.i. Autoradiography, fluorescence imaging, and HE staining were sequentially performed in the same tumor section. b HE staining; c 64Cu autoradiogram in green; d Merged image of b and c; e Cy5.5 fluorescence in red; f Merged image of b and e; g Merged image of b, c, and e. Yellow, red/green overlay. The necrotic region is marked by a dotted line. Scale bar, 2 mm

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