Fig. 6

Representative examples of ex vivo images obtained from the same glioblastoma tumor extracted from a nude mouse after sacrifice showing (1) the distribution of ⁶⁸Ga-NODAGA-RGD recorded by the μImager™ and (2) with several immunohistochemical staining applied on contiguous slices for a morphological analysis (hematoxylin-eosin) and for analyzing the expressions of the αv subunit and the Ki-67 protein (an indicator of cell proliferation). Note that the expressions of the αv subunit (third panel c2 bottom) and of the Ki-67 (last panel bottom d2 right) protein appear much higher in a selected area showing a high level of recorded ⁶⁸Ga-NODAGA-RGD counts on the μImager™ (15.240 counts-per-minute, images from the lower third of the figure; first panel bottom left), when compared with another area showing a low level of recorded counts (9.878 counts-per-minute, images from the middle third of the figure; first panel middle). By contrast, cell density appears equivalent in these two areas (second panel b1 and b2 middle and bottom) on the hematoxylin-eosin stained images