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Fig. 4 | EJNMMI Research

Fig. 4

From: Effect of starvation on brain glucose metabolism and 18F-2-fluoro-2-deoxyglucose uptake: an experimental in-vivo and ex-vivo study

Fig. 4

STS effect on determinants of glucose and FDG entrapment. a The western blot analysis against GLUT1, GLUT2, GLUT3, and GLUT4 performed in brain homogenate of control (CTR, green bars) and of starved mice (STS, blue bars). Protein levels of the different GLUT isoforms were remarkably stable regardless of nutritional status. Data were expressed in percentage change over control ± SD of at least three experiments. The values have been normalized on β-actin. b The HK gene expression profiling of brain homogenates in CTR (left) and STS mice (right) was obtained by real-time PCR. The four different isoforms have been analyzed: HK I (blue bar), HK II (red bar), HK III (green bar), and HK IV (purple bar). Results are expressed in mean normalized expression ± SD. Nutritional status did not affect this expression profile. c The HK activity of brain homogenate in CTR mice (green bar) and in STS mice (blue bar) in the presence of glucose (left) and 2DG (right). Data are expressed in μmol × min−1 ± SD of at least three experiments. d HK Km expressed determined by Lineweaver-Burk double reciprocal plots. Data are expressed in mM ± SD of at least three experiments. e, f the western blot analysis against G6Pase and the G6Pase activities, respectively. In both graphs, CTR group is represented in blue and the STS mice in green. Western blot results are expressed in percentage change over control ± SD of at least three experiments. Values have been normalized on β-actin. The G6Pase activity was assayed in the presence of glucose-6P (left) and 2DG-6P (right), and the values are expressed in U × mg of proteins−1 ± SD of at least three experiments

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