Parameter | Control 3 weeks | CDAHFD 3 weeks | Control 8 weeks | CDAHFD 8 weeks |
|---|
Steatosis score | 0.00 ± 0.00 | 1.75 ± 0.71** | 0.00 ± 0.00 | 1.88 ± 0.35** |
Inflammation score | 0.00 ± 0.00 | 2.88 ± 0.35** | 0.00 ± 0.00 | 3.00 ± 0.00** |
Ballooning score | 0.00 ± 0.00 | 0.38 ± 0.52** | 0.00 ± 0.00 | 0.88 ± 0.64** |
NAFLD activity score | 0.00 ± 0.00 | 5.00 ± 1.07** | 0.00 ± 0.00 | 5.75 ± 0.71** |
Fibrosis area (%) | 0.61 ± 0.29 | 0.47 ± 0.19 | 0.65 ± 0.18 | 3.89 ± 1.81**## |
- Representative photomicrographs of hepatic histology stained with hematoxylin and eosin (H&E) and Sirius red. Steatosis and inflammation scores ranged from 0 to 3 (normal = 0; minimal = 1; moderate = 2; marked = 3). Ballooning score ranged from 0 to 2 (normal = 0; minimal = 1; marked = 2). NAFLD activity score (NAS) was calculated by using the sum of each histological score. Data are expressed as the mean ± SD (n = 8 mice per group). Statistical differences were assessed using Steel-Dwass test. Hematoxylin and eosin (H&E) and Sirius red, × 200 magnification
- *p < 0.05, **p < 0.01 compared with respective control mice. ##p < 0.01 compared with CDAHFD 3 weeks
