Table 1 Primer sequences used for qPCR analysis
Gene | NCBI reference sequence | Forward (5′→3′) | Reverse (3′→5′) | Amplicon size (base pairs) |
|---|---|---|---|---|
GAPDH | NM_002046.3 | TCAAGGGCATCCTGGGCTAC | CGTCAAAGGTGGAGGAGTGG | 81 |
TGM1 | NM_000359.2 | CAATGTCTCAGGCCACGTC | CCAGTAACGTGAGGGAGAGG | 95 |
TGM2 | NM_004613.2 | AGAGGAGCGGCAGGAGTATG | AGGATCCCATCTTCAAACTGC | 111 |
TGM3 | NM_003245.3 | AACCTGAAGATCGACGTGC | CAGTTGCTTGGTGCCACTC | 93 |
TGM5 | NM_201631.3 | TCCTGGTGAACAAGATCATC | GTATGGAGAGTGGCTGGTTC | 90 |
F13A1 | NM_000129.3 | TCCGCAGAGGGCAGTCTTTC | CCTGTGGGTAGCGACCAATGA | 105 |