Fig. 3

Experimental scheme for pretargeted imaging studies. Both mAb groups (A and B) and the nonspecific IgG control received the same mass of antibody via lateral tail vein injection. In the mAb pretargeting experiments, the same total molar amount of non-radioactive TAF was given to each animal after the designated lag time of 24, 48, or 72 h either by mixing it directly to the [¹⁸F]TAF injection and reducing its molar activity (group A, n = 4) or by giving the non-radioactive TAF 5 min prior to administration of [¹⁸F]TAF (group B, n = 4). The lag time of 72 h and the single administration of the tracer was chosen for the experimental setup for nonspecific IgG control (n = 5) corresponding to the conditions in which the mAb pretargeting was the most successful