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Fig. 2 | EJNMMI Research

Fig. 2

From: Simple, mild, one-step labelling of proteins with gallium-68 using a tris(hydroxypyridinone) bifunctional chelator: a 68Ga-THP-scFv targeting the prostate-specific membrane antigen

Fig. 2

Size-exclusion HPLC of THP-mal-J591-scFv after radiolabelling with gallium-68 (SEC-2000, column, flow rate 1 ml/min, UV detection at 280 nm). Top: UV chromatogram, showing THP-mal-J591-scFv eluting at ca. 8.5 min and its non-covalent or disulfide-bonded dimer eluting at 7.8 min; bottom: simultaneously recorded radiochromatogram, showing radioactivity associated with THP-J591c-scFv (8.5 min, 84%), dimer (7.8 min, 14%) and unbound gallium-68 (10–12 min, < 2.6%). A small radioactive higher molecular weight protein was also observed (5.5 min, < 2.5%)

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