Fitting approach | k on [M−1 min−1] | k off [min−1] | K d [nM] | R 2 | df |
---|---|---|---|---|---|
2 concentrations | 8.675 × 107 ± 0.414 × 107 | 0.077 ± 0.008 | 0.887 ± 0.121 | 0.992 | 216 |
3 concentrations | 9.262 × 107 ± 0.835 × 107 | 0.049 ± 0.007 | 0.528 ± 0.118 | 0.991 | 325 |
4 concentrations | 9.243 × 107 ± 0.919 × 107 | 0.051 ± 0.007 | 0.556 ± 0.132 | 0.988 | 434 |
5 concentrations | 8.805 × 107 ± 0.289 × 107 | 0.062 ± 0.003 | 0.704 ± 0.054 | 0.994 | 543 |
6 concentrations | 8.788 × 107 ± 0.292 × 107 | 0.061 ± 0.003 | 0.695 ± 0.052 | 0.993 | 652 |
7 concentrations | 8.105 × 107 ± 0.259 × 107 | 0.055 ± 0.003 | 0.683 ± 0.056 | 0.993 | 761 |
8 concentrations | 8.068 × 107 ± 0.260 × 107 | 0.057 ± 0.003 | 0.711 ± 0.056 | 0.993 | 870 |
Averaged | 6.645 × 107 ± 1.423 × 107 | 0.043 ± 0.014 | 0.636 ± 0.076 | nd | nd |
Reported affinity | nd | nd | 0.59 ± 0.02 [29] | nd | nd |