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Table 1 Estimates of the K i values determined in competitive real-time cell-binding experiments

From: New approaches for the reliable in vitro assessment of binding affinity based on high-resolution real-time data acquisition of radioligand-receptor binding kinetics

  1. Displacement of specific [125I]-AB-MECA binding on CHO-K1-hA3R cells at ambient temperature in presence of different A3R antagonists with corresponding HPLClogD7.4 parameters. Reported affinity values were determined with traditional binding assays using membranes from human A3R-transfected HEK-293 cells. Data in the table are expressed as mean ± SEM from three independent experiments
  2. nd not determined