Fig. 3From: New approaches for the reliable in vitro assessment of binding affinity based on high-resolution real-time data acquisition of radioligand-receptor binding kineticsRepresentative association kinetics of [125I]-AB-MECA. Binding association of 0.7 nM [125I]-AB-MECA at ambient temperature to CHO-K1-hA3R cells (a) and to CHO-K1 cells, without the dedicated target receptor (b). Differences were proved to be statistically significant (P < 0.0001) using two-way repeated measures ANOVA with Sidak’s multiple comparisons test. Data are expressed as mean ± SEM from three independent experiments. If not visible, error bars are within the margin of the symbolsBack to article page