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EJNMMI Research
Fig. 2 | EJNMMI Research

Fig. 2

From: New approaches for the reliable in vitro assessment of binding affinity based on high-resolution real-time data acquisition of radioligand-receptor binding kinetics

Fig. 2

Representation of the measurement protocol. Real-time binding data of both experimental approaches were acquired according to repeated measurements of six dedicated reading points on a rotating petri dish (a). Measured points 1, 2 and 6 were located in the cell-free area (reference pole), whereas 3, 4 and 5 were set on the area in which the cells were seeded (cell pole). Data from each reading point were acquired over 4 s with a delay of 2 s between successive measurements. The corresponding radioactive signal with respect to the dedicated measurement point was monitored continuously in the perimeter trace (b). Representative graph from one experiment. Data are shown as mean ± SEM from eight consecutive measurements at binding equilibrium. If not visible, error bars are within the margin of the symbols

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