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EJNMMI Research
Fig. 1 | EJNMMI Research

Fig. 1

From: New approaches for the reliable in vitro assessment of binding affinity based on high-resolution real-time data acquisition of radioligand-receptor binding kinetics

Fig. 1

Schematic illustration of the petri dish preparation. 106 CHO-K1-hA3R cells were seeded on a local part a tilted petri dish and incubated in 2 mL of medium for 24 h (a). On the consecutive day, the petri dish was preserved in a horizontal position and the cells were cultured with 10 mL of fresh medium for additional 24 h (b). On the third day, the petri dish was subjected to the binding experiment and the cells were provided with 3 mL of medium without additives (c)

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