Fig. 1

Experimental workflow. GOT1-bearing BALB/c nude mice were divided into three studies: one biodistribution and dosimetry study and two therapeutic studies. In these studies, tumor-bearing mice received either a single injection of ¹⁷⁷Lu-octreotate or a priming administration followed by a subsequent injection of ¹⁷⁷Lu-octreotate 24 h later. Magnetic resonance imaging (MRI), biokinetics, mean absorbed dose, tumor volume, tumor morphology, and gene expression of tumors were analyzed. Dagger symbol indicates that animals were killed and dissected; yellow indicates that radioactivity measurements and dosimetric calculations were performed on samples from adrenals, blood, kidneys, liver, lungs, pancreas, spleen, and tumor; blue indicates that tumor samples were fixed in formaldehyde, embedded in paraffin, and subjected to morphological and immunohistochemical analyses; green indicates that tumor samples were snap frozen in lN₂ followed by RNA extraction and gene expression analysis