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EJNMMI Research
Fig. 1 | EJNMMI Research

Fig. 1

From: Cryopreservation of primary human monocytes does not negatively affect their functionality or their ability to be labelled with radionuclides: basis for molecular imaging and cell therapy

Fig. 1

Effects of cryopreservation on viability of CD14++CD16− monocytes. a Recovery of cryopreserved human monocytes after thawing. b Freshly isolated and cryopreserved cells were analysed for their survival using a trypan blue exclusion method. Relative ratios of the alive-to-dead freshly isolated and cryopreserved monocytes were calculated. c–e Freshly isolated and cryopreserved cells were analysed with the MTS viability assay at 0 h, 24 h after isolation or thawing, 2–4 weeks (n = 7) or 4–5 weeks (n = 10) after cryopreservation. Statistics: Kruskal-Wallis test with Dunn’s post test, p value: *p < 0.05

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