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Fig. 4 | EJNMMI Research

Fig. 4

From: Preclinical PET imaging of EGFR levels: pairing a targeting with a non-targeting Sel-tagged Affibody-based tracer to estimate the specific uptake

Fig. 4

Ex vivo analyses of sections of the two tumors in Fig. 3b (left tumor (ac); right tumor (df)). a, d Phosphoimaging of sections of tumors excised immediately after the 60 min PET scan with [methyl-11C]-ZEGFR:2377-ST-CH3. b, e IHC detecting CAIX, staining was performed on formalin-fixed, paraffin-embedded material using antibody against CAIX (BioScience, Slovakia) at a dilution 1:500. c, f IHC detecting EGFR, staining was performed on formalin-fixed, paraffin-embedded material using antibody against EGFR (Sigma, Sweden) at a dilution 1:800

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