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Fig. 1 | EJNMMI Research

Fig. 1

From: PET imaging of cyclooxygenase-2 (COX-2) in a pre-clinical colorectal cancer model

Fig. 1

a Radiosynthesis of [18F]Pyricoxib utilizing building block 4-[18F]fluoro-benzylamine ([18F]FBA). b Western blot analysis of COX-1 and COX-2 in cell lysates of HCA-7 and HCT-116 cell lines. c Uptake of [18F]Pyricoxib into HCA-7 and HCT-116 cells over 90 min. Data are normalized as %radioactivity per mg protein and shown as mean ± SEM from n = 3 experiments. d Blocking of [18F]Pyricoxib uptake (60-min incubation time) into HCA-7 cells using high concentrations (0.1 mM) of different selective COX-2 inhibitors. Data are normalized as % radioactivity per mg protein and shown as mean ± SEM from nine data points out of three experiments. e Concentration dependent inhibition of [18F]Pyricoxib uptake (60-min incubation time) into HCA-7 cells. Data are normalized as % maximum uptake of [18F]Pyricoxib and analyzed as mean ± SEM from nine data points out of three experiments. *P < 0.05; ***P < 0.001

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