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EJNMMI Research
Fig. 5 | EJNMMI Research

Fig. 5

From: Fluorescence imaging of in vivo miR-124a-induced neurogenesis of neuronal progenitor cells using neuron-specific reporters

Fig. 5

In vivo fluorescence imaging in the differentiated F11 cells induced by miR-124a. F11 cells transfected with pTα1-RFP were subcutaneously inoculated into both thighs of nude mice with matrigel (n = 3). Before injection, F11 cells were mixed with miRNAs (miR-124a or miR-scramble) coated with in vivo-jetPEI™ solution. a Fluorescence images were daily acquired in individual group up to 4 days. RFP signals in miR-124a-treated F11 cells were higher in nude mice at 4 days, compared to those in miR-scramble-treated F11 cells. b After the mouse skin was removed at 4 days, ROI analysis demonstrated higher fluorescence signals in miR-124a-treated cells than miR-scramble-treated cells (n = 3 *p < 0.05). ROI area was drawn on each injection site. c F11 cells were isolated from the injection sites from the sacrificed nude mice, and immunohistochemistry was done for RFP and neuronal markers (Tuj1 and NF). MiR-124a-treated F11 cells showed increased RFP, Tuj1, and NF levels, compared with miR-scramble-treated group

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