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Table 1 Immunoreactive fraction (% total binding) of hu3S193 antibodies incubated in human serum at 37 °C for 6 days

From: Engineering anti-Lewis-Y hu3S193 antibodies with improved therapeutic ratio for radioimmunotherapy of epithelial cancers

Radiolabel

Antibody

D0

D2

D6

125I-

Wild type

78.64 ± 1.59a

59.09 ± 5.54

32.36 ± 0.12

 

I253A

66.76 ± 0.33

47.50 ± 2.69

30.99 ± 0.77

 

H310A

70.75 ± 3.99

53.52 ± 3.53

32.95 ± 0.28

 

H435A

69.50 ± 0.33

54.59 ± 4.68

34.82 ± 2.98

 

I253A/H310A

71.93 ± 3.22

58.29 ± 0.33

30.52 ± 1.45

111In-

Wild type

86.84 ± 0.74

64.46 ± 8.04

40.21 ± 0.62

 

I253A

83.47 ± 4.26

59.16 ± 3.05

40.77 ± 0.53

 

H310A

81.03 ± 3.38

55.83 ± 1.78

35.64 ± 0.29

 

H435A

83.38 ± 3.81

59.70 ± 7.27

46.19 ± 4.63

 

I253A/H310A

86.07 ± 2.74

57.18 ± 11.61

42.54 ± 1.15

  1. aData are presented as average ± SD (D2 125I-, n = 2; D6 125I- and 111In-, n = 2; rest n = 4)