Fig. 3

Ex vivo SPECT/CT images (upper row) and the corresponding oil red o staining (bottom row) of the aorta and carotids of ApoE KO-cuff mice fed a high-fat diet (a, b), ApoE KO animals fed a high-fat diet (c), ApoE KO animals fed a normal diet (d), and C57BL/6 mice fed a normal diet (e). Animals were injected intravenously with ~10 MBq ¹¹¹In-DOTA-belatacept and sacrificed 48 h p.i. b Blockade with co-injection of an excess of unlabeled belatacept. Color scales for relative % ID/g. Scale bars 1 mm. ApoE KO-cuff mice (a, b) had flow-altering devices implanted around their carotids visible as transparent or reddish material fixed with a black suture (asterisks). a and b were directly compared in one scan, c, d, and e in a second scan (color scales may differ between the two panels)