Fig. 1

17G1 cross-reactivity with canine PSMA. a Protein extracts of PCa cell lines, human (PC-3 and LNCaP) and canine (DPC-1), probed with 17G1 and J591 in dot (top left) and Western (top middle) blots and also submitted to immunoprecipitation of PSMA in DPC-1 and LNCaP protein extracts and SDS-PAGE gels to stain proteins with silver nitrate (top right). Arrow indicates the PSMA position at ~100 kDa. PSMA expression in DPC-1 cells, as detected by immunofluorescence (IF) with 17G1 (red staining; lower left) and DAPI nuclear staining (in blue; lower right). b PSMA expression by IHC with 17G1 in the canine prostate (top left) and primary DPC-1 tumor (top right), human LNCaP tumor xenograft (lower left), benign human prostate (lower middle), and PCa tissue (lower right). Arrow indicates unstained endothelial cells