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Animal model
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Mice originally used for various oncology studies
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Human HCT116 colon cancer xenografted nude mice (female, Charles River Laboratory)
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Albino mice (males, 15 to 20 g, Swiss CD-1(ICR)BR, Charles River Laboratory)
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Number of animals
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50
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12
|
30
|
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Injection method
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In-house needle catheters 30-gauge needles insert into 15 cm of polyethylene 10 tubing with a Blunt 30-gauge Luer-lock hub
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29.5-gauge Terumo insulin syringe
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Needle without precision
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Tracer
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18F-FDG
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18F-FPP(RGD)2
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Mixed of two reagents: 99mTc-EB1 and colloid gold
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|
Injected activity
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2–9 MBq over 20–30 s
|
1.9–3.8 MBq
|
-
|
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Injected volume
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170 μL
|
100 μL
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100 μL of mixture
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Misinjection frequency and assessment
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7/50 (14.0 %)
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4/23 (17.4 %)
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12/30 (40.0 %)
|
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Intermediate or poor injection based on qualitative assessment
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Visual inspection
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Injection efficiency <90 % based on quantitative assessment
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Quantitative evaluation
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Mean %ID/g on tail ranging from 2.4 to 28.4
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Percentage-intended dose not injected ranging from 0.5 to 9.1 %
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Percentage-intended dose not injected ranging from 12 to 63 %
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