Fig. 1

Tumor processing. a The rat was anesthetized, transverse markings were drawn across the tumor (circled in yellow), and fiducial markers (white squares) were placed. b After the whole rat was frozen, the portion of the rat containing tumor (with tumor circled in yellow) was sectioned into transverse blocks along the skin markings and placed into an airtight container. The individual sections were then frozen and then analyzed visually for viability with preservation of orientation