Table 1 Antibody characteristics and antigen expression
Antigen | Clone (supplier) | Dilution | Antigen expression on tumor-infiltrating cells |
|---|---|---|---|
CD2 | OX-35 (AbD Serotec) | 1:800 | T-cells, B-cells, NK cells |
CD3 | Polyclonal (18–0102, Invitrogen) | 1:200 | T-cells |
CD8α | OX-8 (AbD Serotec) | 1:200 | Cytotoxic T-cell, NK cells, DC subset |
CD68 | ED-1 (AbD Serotec) | 1:200 | All macrophages, neutrophils, DC, myeloid progenitors (e.g. myeloid-derived suppressor cell) |
CD163 | ED-2 (LSBio, LifeSpan BioSciences) | 1:200 |