Imaging of reactive oxygen species in focal ischemic mouse brain using a radical trapping tracer [3H]hydromethidine

Abe, Kohji; Tonomura, Misato; Ito, Miwa; Takai, Nozomi; Imamoto, Natsumi; Rokugawa, Takemi; Momosaki, Sotaro; Fukumoto, Kazumi; Morimoto, Kenji; Inoue, Osamu

doi:10.1186/s13550-015-0115-1

EJNMMI Research

Table 1 Radioactivity concentration of the brain after intravenous injection of [³H]hydromethidine to tMCAO mice treated with saline and DMTU

From: Imaging of reactive oxygen species in focal ischemic mouse brain using a radical trapping tracer [³H]hydromethidine

Brain region			CTX-1 (Bq/mg)	CTX-2 (Bq/mg)	CTX-3 (Bq/mg)	STR-1 (Bq/mg)	STR-2 (Bq/mg)	HIP (Bq/mg)	TH (Bq/mg)
Control		Right	30.5 ± 2.7	30.7 ± 3.0	29.1 ± 1.3	30.5 ± 2.3	31.2 ± 2.5	30.6 ± 2.7	35.3 ± 3.6
Control		Left	30.2 ± 2.7	31.0 ± 2.8	29.6 ± 2.5	30.3 ± 2.5	31.2 ± 2.7	30.2 ± 3.7	34.9 ± 3.9
tMCAO + Saline	1 h	Right (C)	25.9 ± 2.0	26.1 ± 1.2	26.3 ± 2.0	26.5 ± 1.4	27.0 ± 1.5	29.9 ± 2.1	29.8 ± 2.5
	1 h	Left (I)	28.0 ± 1.6	29.1 ± 1.1	33.4 ± 5.0	41.9 ± 7.2	64.4 ± 17.2	34.8 ± 6.0	32.7 ± 4.6
	2 h	Right (C)	37.9 ± 9.1	37.6 ± 8.9	37.0 ± 8.3	39.1 ± 9.0	39.3 ± 9.5	43.6 ± 12.0	42.2 ± 8.8
	2 h	Left (I)	36.4 ± 6.5	36.9 ± 6.8	37.0 ± 7.3	44.9 ± 23.8	50.4 ± 31.4	49.1 ± 9.5	40.7 ± 5.5
	5 h	Right (C)	21.4 ± 2.6	21.3 ± 2.2	20.9 ± 2.4	22.1 ± 2.9	22.7 ± 2.9	23.8 ± 2.8	24.4 ± 2.8^*
	5 h	Left (I)	31.1 ± 7.2	30.0 ± 6.5	28.7 ± 10.0	62.5 ± 18.5^*	68.4 ± 19.8^*	49.7 ± 20.2	34.2 ± 12.4
	7 h	Right (C)	36.2 ± 5.5	36.0 ± 5.5	35.6 ± 5.4	37.7 ± 5.8	38.1 ± 5.7	40.3 ± 7.5	41.6 ± 4.6
	7 h	Left (I)	42.6 ± 11.0	44.7 ± 14.1^*	48.3 ± 13.5^**	64.7 ± 15.5^**	72.7 ± 18.2^**	62.2 ± 21.2^**	49.5 ± 10.6^*
tMCAO + DMTU	1 h	Right (C)	34.6 ± 7.7	34.7 ± 7.1	33.5 ± 5.8	35.2 ± 6.6	36.1 ± 7.2	41.4 ± 8.3	38.3 ± 7.6
	1 h	Left (I)	33.1 ± 6.8	33.5 ± 5.8	35.2 ± 7.4	34.0 ± 9.8	42.8 ± 20.6	43.7 ± 15.1	38.2 ± 6.9
	5 h	Right (C)	38.6 ± 4.9	38.6 ± 4.7	38.1 ± 4.5	40.8 ± 5.9		49.2 ± 7.3^**	44.3 ± 5.8
	5 h	Left (I)	46.4 ± 13.5^*	46.5 ± 10.0^*	46.0 ± 11.6^*	62.5 ± 19.9^*	69.1 ± 18.8^*	51.1 ± 12.0	41.3 ± 4.2
	7 h	Right (C)	62.4 ± 10.5^**	61.6 ± 10.0^**	61.2 ± 9.5^**	64.8 ± 11.4^**	64.4 ± 11.6^**	78.3 ± 14.0^**	66.9 ± 10.9^**
	7 h	Left (I)	63.8 ± 10.7^**	64.3 ± 10.1^**	64.2 ± 8.3^**	79.0 ± 19.5^**	80.6 ± 19.6^**	76.7 ± 15.1^**	65.8 ± 11.2^**

[³H]Hydromethidine was intravenously injected into mice treated with saline or DMTU (200 mg/kg, i.p.) 30 min before tMCAO (90 min). Coronal sections at the level of the striatum and hippocampus were prepared at 1, 2, 5, and 7 h after ischemia/reperfusion of MCA in mice. Data are expressed as mean ± SD (Bq/mg, n = 5–8 for each time point)
C contralateral, I ipsilateral
*P < 0.05, **P < 0.01, as compared with control mice

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