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Table 1 Radioactivity concentration of the brain after intravenous injection of [3H]hydromethidine to tMCAO mice treated with saline and DMTU

From: Imaging of reactive oxygen species in focal ischemic mouse brain using a radical trapping tracer [3H]hydromethidine

Brain region CTX-1 (Bq/mg) CTX-2 (Bq/mg) CTX-3 (Bq/mg) STR-1 (Bq/mg) STR-2 (Bq/mg) HIP (Bq/mg) TH (Bq/mg)
Control Right 30.5 ± 2.7 30.7 ± 3.0 29.1 ± 1.3 30.5 ± 2.3 31.2 ± 2.5 30.6 ± 2.7 35.3 ± 3.6
Left 30.2 ± 2.7 31.0 ± 2.8 29.6 ± 2.5 30.3 ± 2.5 31.2 ± 2.7 30.2 ± 3.7 34.9 ± 3.9
tMCAO + Saline 1 h Right (C) 25.9 ± 2.0 26.1 ± 1.2 26.3 ± 2.0 26.5 ± 1.4 27.0 ± 1.5 29.9 ± 2.1 29.8 ± 2.5
Left (I) 28.0 ± 1.6 29.1 ± 1.1 33.4 ± 5.0 41.9 ± 7.2 64.4 ± 17.2 34.8 ± 6.0 32.7 ± 4.6
2 h Right (C) 37.9 ± 9.1 37.6 ± 8.9 37.0 ± 8.3 39.1 ± 9.0 39.3 ± 9.5 43.6 ± 12.0 42.2 ± 8.8
Left (I) 36.4 ± 6.5 36.9 ± 6.8 37.0 ± 7.3 44.9 ± 23.8 50.4 ± 31.4 49.1 ± 9.5 40.7 ± 5.5
  5 h Right (C) 21.4 ± 2.6 21.3 ± 2.2 20.9 ± 2.4 22.1 ± 2.9 22.7 ± 2.9 23.8 ± 2.8 24.4 ± 2.8*
  Left (I) 31.1 ± 7.2 30.0 ± 6.5 28.7 ± 10.0 62.5 ± 18.5* 68.4 ± 19.8* 49.7 ± 20.2 34.2 ± 12.4
  7 h Right (C) 36.2 ± 5.5 36.0 ± 5.5 35.6 ± 5.4 37.7 ± 5.8 38.1 ± 5.7 40.3 ± 7.5 41.6 ± 4.6
  Left (I) 42.6 ± 11.0 44.7 ± 14.1* 48.3 ± 13.5** 64.7 ± 15.5** 72.7 ± 18.2** 62.2 ± 21.2** 49.5 ± 10.6*
tMCAO + DMTU 1 h Right (C) 34.6 ± 7.7 34.7 ± 7.1 33.5 ± 5.8 35.2 ± 6.6 36.1 ± 7.2 41.4 ± 8.3 38.3 ± 7.6
Left (I) 33.1 ± 6.8 33.5 ± 5.8 35.2 ± 7.4 34.0 ± 9.8 42.8 ± 20.6 43.7 ± 15.1 38.2 ± 6.9
5 h Right (C) 38.6 ± 4.9 38.6 ± 4.7 38.1 ± 4.5 40.8 ± 5.9   49.2 ± 7.3** 44.3 ± 5.8
Left (I) 46.4 ± 13.5* 46.5 ± 10.0* 46.0 ± 11.6* 62.5 ± 19.9* 69.1 ± 18.8* 51.1 ± 12.0 41.3 ± 4.2
7 h Right (C) 62.4 ± 10.5** 61.6 ± 10.0** 61.2 ± 9.5** 64.8 ± 11.4** 64.4 ± 11.6** 78.3 ± 14.0** 66.9 ± 10.9**
Left (I) 63.8 ± 10.7** 64.3 ± 10.1** 64.2 ± 8.3** 79.0 ± 19.5** 80.6 ± 19.6** 76.7 ± 15.1** 65.8 ± 11.2**
  1. [3H]Hydromethidine was intravenously injected into mice treated with saline or DMTU (200 mg/kg, i.p.) 30 min before tMCAO (90 min). Coronal sections at the level of the striatum and hippocampus were prepared at 1, 2, 5, and 7 h after ischemia/reperfusion of MCA in mice. Data are expressed as mean ± SD (Bq/mg, n = 5–8 for each time point)
  2. C contralateral, I ipsilateral
  3. *P < 0.05, **P < 0.01, as compared with control mice